Biochemical Study of the partially Purified Xanthine Oxidase from Sera of Patients with Vitamin D3 Deficiency
Keywords:
انزيم الزانثين، الوبيورينول، فيتامين D3، التثبيط اللاتنافسيAbstract
تم في هذه الدراسة تحضير مركب مشتق من عقار الوبيورينول لدراسة تأثيره على إنزيم زانثين اوكسيديز المنقى جزئيا من مصل دم المرضى المصابين بنقص فيتامين D3 حيث تم تنقية الإنزيم جزئيا ودراسة حركيات الإنزيم كما تم دراسة تأثير عقار الوبيورينول والمركب المحضر المشتق من العقار على فعالية الإنزيم المنقى حيث أظهرت النتائج إن قيمة السرعة القصوى 2.5 U/L وقيمة ثابت ميكاليس منتن هي 5.88×10-5 مولاري بينما كانت درجة الحرارة المثلى للإنزيم 37 مº وقيمة pH = 7.5 . تم اختبار تأثير المركب المحضر وتبين إن المركب يثبط فعالية الإنزيم وان نسبة التثبيط تزيد كلما ازداد تركيز المركب وكان نوع التثبيط من النوع اللاتنافسي Uncompetitive inhibition
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