Evaluating the Antimicrobial Efficacy of Apple Cider Vinegar on Bacteria and Fungi Isolated from Vaginitis
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Abstract
Vaginitis is a prevalent medical illness that affects a substantial proportion of women, mostly attributed to the excessive proliferation of bacteria and fungi. The objective of this study was to assess the antimicrobial effectiveness of Apple Cider Vinegar against bacteria and fungi that were obtained from cases of vaginitis. 50 vaginal swabs were collected from vaginitis patients at private clinics in Kirkuk City, Iraq (10 pregnant women and 40 non-pregnant women). The research included the isolation and identification of bacterial and fungal isolates. All isolates performed the Minimum Inhibitory Concentration and disc diffusion techniques tests. Different concentrations of apple cider vinegar (20%, 30%, 40%, 50% and 100%) were tested against the isolates and Standard antibiotic drugs were used as positive controls. The results of 50 patients were examined, showing that 22 (44%) had bacterial vaginitis and 39 (78%) had fungal vaginitis. Vaginitis was more prevalent in pregnant women compared with non-pregnant. Staphylococcus aureus recorded 14 (24.9%) out of the 54 bacterial isolates, followed by Echerichia coli, Group B- Streptococcus, Klebsiella pneumoniae, Staphylococcus epidermidis and Pseudomonas aeruginosa respectively. whereas Candida albicans recorded 30 (61.2%) out of the 49 fungal isolates, followed by Candida tropicalis and Candida parapsilosis, respectively. The findings showed a range of sensitivity to apple cider vinegar among the isolated samples. The efficacy of apple cider vinegar was found to be greater against fungal isolates compared to bacterial isolates, with a higher effectiveness observed against gram-negative bacteria in comparison to gram-positive bacteria. The highest MIC values were found for Gram-positive bacteria, which recorded 125 (µg/mL), followed by Gram-negative bacteria, 62.5 μg/ml. While the lowest MIC values for the isolated fungi were (31.25 μg/ml). A comparison to traditional antimicrobial drugs has highlighted ACV's potential as an alternative treatment for vaginitis. According to these findings, ACV may be an effective treatment choice for vaginitis.
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Egan ME, Lipsky MS. Diagnosis of vaginitis. American family physician. 2000; 62(5):1095-1104. https://www.scirp.org/reference/referencespapers?referenceid=1196881
Joesoef M, Schmid G, Hillier S. Bacterial vaginosis: review of treatment options and potential clinical indications for therapy. Clinical Infectious Diseases. 1999; 28(Supplement_1):S57-S65. DOI: 10.1086/514725
Stenchever MA. Comprehensive gynaecology .4th ed. Mosby Inc; 2001; (4):668–6678. https://www.amazon.com/Comprehensive-Gynecology-Mishell Herbst/dp/032301402X
Sobel JD. Vaginitis. New England Journal of Medicine. 1997; 337(26):1896-1903. DOI: 10.1056/NEJM199712253372607
Tacconelli E. Global Priority List of Antibiotic-Resistant Bacteria to Guide Research, Discovery, and Development. 2017. https://remed.org/wp-content/uploads/2017/03/lobal-priority-list-of-antibiotic-resistant-bacteria-2017.pdf
Tan S. Vinegar fermentation [Master of Science thesis]. Louisiana State University, Department of Food Science, Baton Rouge p 101s. 2005.
Kalaba V, Balaban Zm, Kalaba D. Antibacterial activity of domestic apple cider vinegar. Agrofor. 2019; 4(1). DOI:10.7251/AGRENG1901024K
Darzi J, Frost G, Montaser R, Yap J, Robertson M. Influence of the tolerability of vinegar as an oral source of short-chain fatty acids on appetite control and food intake. International Journal of Obesity. 2014;38(5):675-681. DOI: 10.1038/ijo.2013.157
Forbes BA, Sahm DF, Weissfeld AS. Diagnostic microbiology: Mosby St Louis; 2007.
Murray, P. R., Baron, E. J., Pfaller, M. A., Tenover, F. C. & Yolken, R. H. Manual of clinical Microbiology.7th ed. ASM press. Wash- ington; 1999.
Baladas B and Altuner Em. The antimicrobial activity of apple cider vinegar and grape vinegar, which are used as a traditional surface disinfectant for fruits and vegetables. Communications Faculty of Sciences University of Ankara Series C Biology. 2018;27(1):1-10. DOI:10.1501/commuc_0000000187
Canli K, Yetgin A, Akata I, Altuner E. Antimicrobial activity and chemical composition screening of Epilobium montanum root. Indian Journal of Pharmaceutical Education and Research. 2017; 51(3). DOI:10.5530/ijper.51.3s.21
Canli K, Altuner Em, Akata I. Antimicrobial screening of Mnium stellare. ||| Bangladesh Journal of Pharmacology. 2015; 10(2):321-325. DOI:10.3329/bjp.v10i2.22463
Hameed SJ. Effect of Metformin on the Efficacy of Antibiotics (In Vitro). European Journal of Molecular and Clinical Medicine. 2022; 9:3235-3241. https://go.gale.com/ps/i.do?p=HRCA&u=googlescholar&id=GALE|A737509216&v=2.1&it=r&sid=googleScholar&asid=ab6763c7
Altuner Em and Canli K. In vitro antimicrobial screening of Hypnum andoi AJE Sm. Kastamonu University Journal of Forestry Faculty. 2012; 12(1):97-101. https://www.researchgate.net/publication/314044097_In_vitro_antimicrobial_screening_of_Hypnum_andoi_AJE_Sm
Altuner E, Çetin B, Çökmüș C. Antimicrobial activity of Tortella tortulosa (Hedw.) Limpr. extracts. Kastamonu Üniversitesi Orman Fakültesi Dergisi. 2010; 10(2):111-116. https://www.researchgate.net/publication/314044097_In_vitro_antimicrobial_screening_of_Hypnum_andoi_AJE_Sm
Altuner EM. Bazı karayosunu türlerinin antimikrobiyal aktivitesinin belirlenmesi. 2008. DOI: 10.26672.anatolianbryology.809057
Hameed S. Effect of Rosmarinus officinalis L. extracts on bacteria isolated from conjunctivitis in Kirkuk City, Iraq. International Journal of Biology Sciences. 2023; 5:87-91. DOI:10.33545/26649926.2023.v5.i1b.150
MA W. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Clsi (Nccls). 2006; 26:M7-A. https://clsi.org/media/1928/m07ed11_sample.pdf
Romanik M, Martirosian G. Frequency, diagnostic criteria and consequences of bacterial vaginosis in pregnant women. Przeglad epidemiologiczny. 2004; 58(3):547-553. https://pubmed.ncbi.nlm.nih.gov/15730019/
Virginia,A.;Rauh,S.C.D.;Jennifer,F.;Culhane,P.H.D.;Vijaya,K.andHogan,D.R.P.H. Bacterial vaginosis :Apublic health problem for woman .J.JAWWA. 2002; 55 (4): 220- 224. https://pubmed.ncbi.nlm.nih.gov/10935356/
Rex, J. H. ; Hwalsh, T. J. ; Sobel, S. G. ; filler, P. G. Pappas, W. E. ; Dismukes,T. W. & Edwards, J. E. Practice guide lines for the treatment of Candidiasis. Clin. Infect. Dis. 2000; 30: 662- 678. DOI: 10.1086/313749
Reid G, Burton J, Hammond J-A, Bruce AW. Nucleic acid-based diagnosis of bacterial vaginosis and improved management using probiotic lactobacilli. Journal of medicinal food. 2004; 7(2):223-228. DOI: 10.1089/1096620041224166
Dybaś I, Sidor-Wójtowicz A, Kozioł-Montewka M. Bacterial flora and mycosis of the vagina in women with symptoms of vaginal inflammation. Ginekologia Polska. 2005; 76(5):385-390. https://www.researchgate.net/publication/7615302_Bacterial_flora_and_mycosis_of_the_vagina_in_women_with_symptoms_of_vaginal_inflammation
Razzak MSA, Al-Charrakh AH, Al-Greitty BH. Relationship between lactobacilli and opportunistic bacterial pathogens associated with vaginitis. North American Journal of Medical Sciences. 2011; 3(4):185. DOI:10.4297/najms.2011.3185
Hussein, R. Comparison of biological and molecular parameters of some Candida species that are sensitive and resistant to some antifungals. Thesis. College of Science. University of Kufa. 2011.DOI: https://doi.org/10.36320/ajb/v15.i2.11882
Yagnik D, Serafin V, J. Shah A. Antimicrobial activity of apple cider vinegar against Escherichia coli, Staphylococcus aureus and Candida albicans; downregulating cytokine and microbial protein expression. Scientific reports. 2018; 8(1):1732. https://www.nature.com/articles/s41598-017-18618-x.
Kavanaugh NL, Ribbeck K. Selected antimicrobial essential oils eradicate Pseudomonas spp. and Staphylococcus aureus biofilms. Applied and environmental microbiology. 2012; 78(11):4057-4061. doi: 10.1128/AEM.07499-11
Tobudic S, Kratzer C, Presterl E. Azole‐resistant Candida spp.–emerging pathogens? Mycoses. 2012; 55:24-32. https://doi.org/10.1111/j.1439-0507.2011.02146.x
Arneborg N, Jespersen L, Jakobsen M. Individual cells of Saccharomyces cerevisiae and Zygosaccharomyces bailii exhibit different short-term intracellular pH responses to acetic acid. Archives of microbiology. 2000; 174:125-128. https://doi.org/10.1007/s002030000185
Guldfeldt LU, Arneborg N. Measurement of the effects of acetic acid and extracellular pH on intracellular pH of nonfermenting, individual Saccharomyces cerevisiae cells by fluorescence microscopy. Applied and environmental microbiology. 1998; 64(2):530-534. DOI: 10.1128/AEM.64.2.530-534.1998
Prudêncio C, Sansonetty F, Côrte‐Real M. Flow cytometric assessment of cell structural and functional changes induced by acetic acid in the yeasts Zygosaccharomyces bailii and Saccharomyces cerevisiae. Cytometry: The Journal of the International Society for Analytical Cytology. 1998; 31(4):307-313. DOI: 10.1002/(sici)1097-0320(19980401)31:4<307::aid-cyto11>3.0.co;2-u
Ousaaid D, Laaroussi H, Bakour M, Ennaji H, Lyoussi B, El Arabi I. Antifungal and antibacterial activities of apple vinegar of different cultivars. International Journal of Microbiology. 2021; 2021. https://doi.org/10.1155/2021/6087671
Patole V, Mahore J, Nandgude and T, Gutte A. Apple cider vinegar: Effective adjuvant treatment for aerobic vaginitis. Novel Research in Microbiology Journal. 2022; 6(4):1659-69. DOI: 10.21608/nrmj.2022.253697