Molecular detection of Virulence Factor Genes in Candida albicans Isolated from the Oral Cavity of Local Chicken and Antifungal Susceptibility in Mosul Province

Abstract

Candida spp. is a naturally occurring bacteria in the GI tracts of numerous species, including birds. Candidiasis is an opportunistic illness that emerges when the normal microflora is disturbed. Clinical findings include thickening mucosa and white, elevated pseudomembranous areas. Gross lesions can serve as a presumptive diagnosis. This study aimed to detect and identify Candida albicans as of oral cavity of chicken. The Seventy samples were gathered using swabbing from the oral cavity of the Chicken and transported to the Mycology laboratory, using a cool container for handling. Samples were inoculated onto sabouraued dextrose agar for presumptive identification of Candida albicans. Based on molecular techniques, regarding the consequences of PCR amplification of the 18S rRNA gene in the identification of Candida albicans, this gene has existed existing 50 samples, Metallo-aminopeptidase gene was present in 50/50 (100%) and the alkaline phosphatase sequence gene was once existing in 50/50 (100%). whilst alpha glucosidase used to be existing in 30/50 (60%) and sterol esterase genes 25/50 (50%). Antifungal sensitivity testing results showed that ketoconazole and itraconazole provided the most exceptional Sensitivity (100%) against Candida albicans isolates, followed by fluconazole and Amphotericin B. The outcomes of the present research showed that local poultry oral cavities likely contain pathogenic yeasts.