Correlation between Vaginal Yeast Infection and Interleukin -10 gene Polymorphism in Women within Mosul City
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Abstract
Vaginal candidiasis is a common condition among women due to genetic and environmental factors, which are associated with the causative agents of Candida species sp, leading to physical symptoms in affected women. Sixty-five samples (45 samples from women with vaginal candidiasis and 20 samples from healthy women) were collected from vaginal swabs and blood samples. Microscopic diagnostic tests were performed to investigate and identify Candida species. Tetra-ARMS PCR was also conducted to determine the association of the interleukin-10 gene polymorphism with vaginal candidiasis in women from the city of Mosul, northern Iraq. Furthermore, genetic variation results were analyzed using five molecular tests. The microscopic examination results of vaginal swabs revealed four types of yeast: Candida albicans represented 67% of sample frequency, C. glabrata 21%, C. tropicalis 7%, while C. krusei represented 5% of sample frequency. Moreover, the results of Tetra-ARMS-PCR of blood samples from infected women showed a higher percentage of the wild CC genotype (70%) compared to the mutant TT genotype (12%), while the heterozygous CT genotype was (18%) compared to the healthy group (55%, 5%, 40% respectively). The frequency of the (C) allele in infected samples was high (80%) compared to the mutant (T) allele (20%), whereas in the healthy group, the frequencies of the C and T alleles were (75% and 25% respectively). The Hardy–Weinberg equilibrium test showed an environmental factor effect and the disappearance of the genetic factor.
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