Baidaa A. The effect of tacrolimus and Triticum Aestivum Germ Oil on the intrinsic pathway of apoptosis in the liver of male albino mice: an immunohistochemical and molecular study

Abstract

Tacrolimus is a commonly used immunosuppressant, while wheat germ oil is a recently discovered compound that is a potent antioxidant. The current study investigated the role of  Triticum Aestivum Germ Oil  in influencing the intrinsic pathway of apoptosis through its effect on the protein Bax.Forty male white mice, Mus musculus were used, divided into four sets, each containing 10 individuals: negative control group (distilled water) set, positive control set (wheat germ oil , 1130 mg/kg), tacrolimus  (0.4 mg/kg) set, and a set treated with tacrolimus and wheat germ oil together. The animals were treated for two periods, 14 and 28 days.   The findings demonstrated that tacrolimus increases the activity of the  Baxdelta2G9 (Bax protein) in hepatocytes; however, after 14 days of treatment with the two chemicals under investigation, the group's immunohistochemistry activity declined, and over the course of 28 days, the response and improvement grew. Additionally, the group that got tacrolimus revealed arise in Bax transcription, whereas the group that received wheat germ oil showed a decrease in this gene expression. Additionally, the improvement was noted as the experiment's duration grew.  We conclude from this that the drug tacrolimus stimulates the activity of the intrinsic pathway of programmed death, but the use of an antioxidant such as wheat germ oil improves and reduces the activity of this pathway in the liver.

Keywords: Cell death, Reactivity, Rodents, Apoptosis, qPCR.