Wael A. O. Antimicrobial Resistance Patterns of Acinetobacter haemolyticus from Urinary Tract Infections and Hospital Wastewater
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Abstract
ABSTRACT: Background: Acinetobacter haemolyticus (A. haemolyticus) is pathogenic species and resistance bacteria to different type of antibiotic and this resistance characteristic return to Abuse of antibiotics and the common gene exchange amongst hospital microorganisms. Objective: The purpose of this research is determine the origin source of Acinetobacter haemolyticus infections and determining the most effective treatment strategy . Methods: Thirty samples used for research and identify A. haemolyticus, fifteen of this samples carried out of urine were placed in sterile urine cups collected from UTIs patient’s and fifteen of samples carried out from hospital wastewater, all samples cultured on Macconkay agar, Eosin methylene blue and blood agar then confirmed the identification through DL microbial ID/AST system (Enterobact. IST CARD) model DL-120E and detected MIC, resistance, suitability of A. haemolyticus for some type of antibiotic. Results: Thirty (30) samples were collected and cultured on different media, diagnosed 5 samples of urine (33,3%) as a positive growth for A, haemolyticus while remaining 10 samples (66.6%) showed negative growth, However, out of 15 hospital wastewater samples, 3 (20%) showed positive growth for A. haemolyticus whereas the remaining 80% showed negative growth. Following confirmation of A.haemolyticus identify using DL microbial ID/AST method at the same time detected MIC, resistance and susceptibility of A. haemolyticus for some type of antibiotic. Found A. haemolyticus was sensitive for some antibiotic include (Ampicillin, Gentamicin, Piperacillin-Tazobactam, Cefepime, Cefuroxime (vi), Trimethoprim-Sulfamethoxazole, Meropenem, Amikacin, Ceftazidime, Cefoperazone-Sulbactam, Minocycline) and resistance for two type of antibiotic include (Imipenem and Meropenem). Conclusion: In this study appeared even though A. haemolyticus was isolated from two distinct sources (sewage and urine) and produced identical results in terms of MIC, antibiotic resistance, and appropriateness, this work opens up a number of other investigations, one of those genes examined each type of bacterium based on where it was isolated (inside or outside the human body), revealing the differences between the bacteria's genes and whether or not environmental factors affected of this bacteria.
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