Iman A. A. Isolation of Gram-negative bacteria from wounds and burn and evaluation of their antibiotic resistance
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Was done in this study isolate and identify Gram-negative bacteria from pus samples collected from patients with wound and burn infections, attending Al-Salam Teaching Hospital, Mosul General Hospital, and Mosul Center for Burns and Plastic Surgery. Bacterial isolates were identified based on cultural, morphological, microscopic characteristics, and biochemical tests. Identification was further confirmed using the VITEK-2 system to ensure accurate species determination. A total of 88 bacterial isolates were obtained from 54 pus samples. Pseudomonas aeruginosa and Enterobacter cloacae were the most prevalent species, accounting for 28 isolates (31.81%). This was followed by Klebsiella pneumoniae with 10 isolates (11.39%), Klebsiella aerogenes with 6 isolates (7%), Escherichia coli with 7 isolates (8%), and Proteus mirabilis, Proteus vulgaris, and Raoultella ornithinolytica with 2 isolates each (2.20%). Additionally, Acinetobacter baumannii, Serratia marcescens, and Salmonella spp. were each represented by 1 isolate (1. 13%). Antibiotics susceptibility testing revealed a high level of resistance among Gram-negative bacteria to cephalosporin antibiotics, with resistance rates of 95.5% for ceftazidime, and 93% for both ceftriaxone and cefotaxime. Moreover, macrolide antibiotics, specifically azithromycin, showed a resistance rate of 75%. In contrast, chloramphenicol demonstrated the highest antibacterial efficacy among the tested antibiotics against Gram-negative isolates.
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