Effect of Silver Nanoparticles Biosynthesized by Chard Leaf Extract (Beta vulgaris) on the Levels of IgM and IgG Immunoglobulins in the Blood of Male Mice
Keywords:
Silver nanoparticles, chard extract, IgM, IgG, male miceAbstract
Background: The basic function of the immune system is to interact، detect، and identify foreign substances such as bacteria، cells، fungi، and parasites and eliminate them in order to protect the host body. Nanoparticles can interfere with and interact with this function، or they can be recognized as antigens of foreign substances and thus stimulate the immune response. Disruption of the immune response can lead to serious medical conditions.
Aim: To study the effect of silver nanoparticles on the concentration level of IgG and IgM immunoglobulins.
Material and methods: This study was conducted in the studies laboratory of the Department of Pathological Analysis/Faculty of Applied Sciences/Samarra University for the period from 4/15/2021 until 6/10/2022. Silver nanoparticles were synthesized using chard leaf extract, and the silver nanoparticles were examined on a large scale. Using ultraviolet and visible spectroscopy (UV-Vis), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), and X-ray scattering (EDX), nanoparticle electrokinetic properties were evaluated using microscopy. Scanning electron microscope (SEM) and transmission electron microscopy (TEM). Twenty male French Albino mice with red eyes were obtained from the General Company for the Manufacture of Pharmaceuticals and Medical Supplies in Samarra. Their weight ranged between 25 and 35 grams. The animals were housed at home. Animals for the College of Applied Sciences/University of Samarra under appropriate environmental conditions and a controlled temperature of 25±5 degrees Celsius while maintaining appropriate light and ventilation, They were divided into two group, and the first group was injected into the peritoneal cavity with a volume of 0.1 ml and a concentration of 250 mg/kg of silver nanoparticle solution for a period of 14 days. The second group was designated as a control group. Blood samples were drawn the next day after the end of the injection period from all study animals. During the slaughter of the animal, after the animals were partially anesthetized with chloroform they were placed in tubes containing gel tubes، and then the tubes were placed in a centrifuge at 5000 rpm for ten particles for the purpose of separating the blood serum from the components of the blood for the purpose of estimating the level of immunoglobulins IgM and IgG using... A special analysis kit was prepared by the American company GLOUD-CLONE CORP using the Sandwich-ELISA method.
Results: The results of the effect of silver nanoparticles on the level of immunoglobulins IgM and IgG showed that there was a significant increase in their levels at the P≤0.05 level، reaching 17.58 and 30.64 pg/ml, respectively, for the group treated with silver nanoparticles، compared to the control group, which reached 10.74 and 9.79 pg/ml, respectively.
Conclusions: A significant increase in the levels of IgG and IgM immunoglobulins in groups of male mice treated with silver nanoparticles compared to the control group.
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