Adiponectin hormone and Measurements of Plasma Membrane Voltage in Isolated Adipocytes of Control and Diabetic Patients

Abstract

Background: Diabetes is one of the most important and social-economic problem worldwide and characterized by serious metabolic, vascular and neurologic complications.
Aim: To isolate adipocytes in diabetic patients and estimate the electrical potential difference (membrane potential) of isolated adipocytes.
Methods: Biopsy of lipid tissue of diabetic patients were taken In Bin-Sina hospital and then adipocytes were isolated in laboratory of College of Science, Baghdad University..
Results: The Adiponectin mean serum value was significantly (P<0.001) higher in diabetic patients (15.60 ± 1.73 g/ml) as compared to controls (5.80 ± 1.22 g/ml). While the measurement of isolated adipocytes current was significantly (P<0.001) different in diabetic patients (-30.4 ± 4 mV) from that in controls ( -61 ± 5mV).
Conclusion: Adipocytes plasma membrane voltage was with significant difference in diabetic patients as compared to controls and this reflect the inflammatory changes at cellular level in diabetes.