Effect of Silver Nanoparticles Biosynthesized by Chard Leaf Extract (Beta vulgaris) on the Levels of Cytokines IL-6, IL-10, and TNF-α in the Blood of Male Mice S
Keywords:
silver nanoparticles, chard extract, IL-6, TNF-α, IL-10, male miceAbstract
Background: There is a high probability that nanoparticles interact within the body with components of the immune system, such as immune cells, which may lead to immune system connections and reactions to nanoparticles. These reactions have the potential to modify immunity, activate or suppress the specific and nonspecific immune response and immune function, and can lead to an inflammatory response, increased receptivity to infectious microbial diseases, persistence of autoimmune diseases, or tumor and cancer progression.
Aim: To study the effect of silver nanoparticles on the levels of the cytokines IL-6, IL-10, and TNF-α.
Material and methods: This study was conducted in the studies laboratory of the Department of Pathological Analysis/Faculty of Applied Sciences/Samarra University for the period from 4/15/2021 until 6/10/2022. Silver nanoparticles were synthesized using chard leaf extract, and the silver nanoparticles were examined on a large scale. Using ultraviolet and visible spectroscopy (UV-Vis), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), and X-ray scattering (EDX), nanoparticle electrokinetic properties were evaluated using microscopy. Scanning electron microscope (SEM) and transmission electron microscopy (TEM). Twenty male French Albino mice with red eyes were obtained from the General Company for the Manufacture of Pharmaceuticals and Medical Supplies in Samarra. Their weight ranged between 25 and 35 grams. The animals were housed at home. Animals for the College of Applied Sciences/University of Samarra under appropriate environmental conditions and a controlled temperature of 25±5 degrees Celsius while maintaining appropriate light and ventilation, They were divided into two groups, and the first group was injected into the peritoneal cavity with a volume of 0.1 ml and a concentration of 250 mg/kg of silver nanoparticle solution for a period of 14 days. The second group was designated as a control group. Blood samples were drawn the next day after the end of the injection period from all study animals. During the slaughter of the animal, after the animals were partially anesthetized with chloroform, they were placed in tubes containing gel tubes, and then the tubes were placed in a centrifuge at 5000 rpm for ten particles for the purpose of separating the blood serum from the components of the blood for the purpose of estimating the level of cytokines IL-6, IL-10, and TNF-α using... A special analysis kit was prepared by the American company GLOUD-CLONE CORP using the Sandwich-ELISA method.
Results: The results of the effect of nanoparticles on the level of cytokines IL-6 and TNF-α showed a significant increase in their levels, reaching 580.40 and 290.43 pg/ml, respectively, and a significant decrease in the level of IL-10 to 102.11 pg/ml for the group treated with silver nanoparticles, compared to the control group of 291.13, 152.84, and 126.49 pg/ml, respectively.
Conclusions: There was a significant increase in IL-6 and TNF-α and a significant decrease in IL-10 in male groups of mice treated with silver nanoparticles compared to the control group.
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