Evaluation of the Levels of Erythropoietin and Some Haehematological Parameters in Patients with History of Haemodialysis and Non- haemodialysis.

Abstract

Background: Kidney diseases are associated  with a variety of hematological changes. Anemia is the most common finding among these haematological indicators and its severity increased rise with disease chronicity.

Aim: To determine the association between erythropoietin and hematology parameters with different stages with chronic kidney failure and haemodialysis.

Subjects and methods: A total of 106 patients with chronic renal failure were included in the study and their age range was (20-69 years) and divided in 5groups according to disease staging. The study comprised 40 participants who had never had dialysis before. While there were 66 patients with a dialysis history, 21 had a dialysis history of less than 6 months and 21 had a dialysis history of more than 6 months (45).Erythropoietin were determined in serum of all subjects by using a commercial ELISA Micro wells kit (My Bio Source, USA). Hematology parameters (reticulocytes, red blood cells, haematocrit and hemoglobin) in whole blood of all subjects were determined by using an automated hematological analyzers XT2000i (Sysmex, Japan).

Results: The erythropoietin concentration, percentages of reticulocyte and red blood cells count were with significant differences (P<0.05)between groups.

Anemia present in all chronic kidney failure patients and increased deterioration directly associated to the stage of kidney failure.

Conclusion: Erythropoietin, reticulocyte, red blood cells, haematocrit and haemoglobin levels were reduced in patients with chronic kidney disease and reduction was associated with disease stages.