Effect of Cladosporium herbarum Extracts on Some Bacterial Species
Plant extract antibacterial activity
Keywords:
Cladosporium herbarum, E. coli, Staph. aureus, secondary metabolitesAbstract
Background: The results of the chemical detection showed that the active groups in the Cladosporium herbarum extracts are Sumiki’s acid and Acetyl Sumiki’s acid, and knowing its biological effectiveness against bacteria
Aim: To evaluate the effect of Cladosporium herbarum extracts on some bacterial species (E. coli & Staph. aureus)
Materials and methods: The fungal extract of Cladosporium herbarum was prepared and tested for its inhibitory effect on Escherichia coli and Staphylococcus aureus. Three concentrations were tested (50, 100, 250 mg/ml). The active compounds of the extract were then identified.
Results: The fungus producing the fungal extract was isolated from various soil samples and was found to be C. herbarum. After growing on the production medium for seven days at 30°C and chemically identifying the active components in the extracts, the presence of sumiki's acid and acetyl sumiki's acid35 in the Cladosporium herbarum fungal extract was found at concentrations of 0.32 and 0.97 mg per 100 ml, respectively. The inhibitory capacity was tested at concentrations of 50, 100, and 250 mg/ml against Staph. aureus and E. coli bacteria. The results showed that the fungal extract varied in its inhibitory effect on the isolated bacteria at a concentration of 50 mg/ml, with an average diameter of inhibition of 13 mm. The average diameter of inhibition at a concentration of 100 mg/ml was 17 and 20 mm, respectively
References
Charya MS. Fungi: An overview. Plant Biology and Biotechnology: Plant diversity, organization, function and improvement. Springer, India. 2015 Jul 2:197-215.
Senesi N, Loffredo E. The chemistry of soil organic matter. In Soil physical chemistry 2018 Feb 6 (pp. 239-370). CRC press.
Aljohani AS. Botanical Compounds: A Promising Approach to Control Mycobacterium Species of Veterinary and Zoonotic Importance. Pakistan Veterinary Journal. 2023 Oct 1;43(4).
Liu D. Classification of medically important fungi. In Molecular Medical Microbiology 2024 Jan 1 (pp. 2763-2777). Academic Press.
Correia J, Borges A, Simões M, Simões LC. Beyond Penicillin: the potential of filamentous fungi for drug discovery in the age of antibiotic resistance. Antibiotics. 2023 Jul 29;12(8):1250.
Lima MA, Oliveira MD, Pimenta AT, Uchôa PK. Aspergillus niger: a hundred years of contribution to the natural products chemistry. Journal of the Brazilian Chemical Society. 2019 Oct 21;30(10):2029-59.
Bills GF, Gloer JB. Biologically active secondary metabolites from the fungi. Microbiology spectrum. 2016 Dec 30;4(6):10-128.
Keller NP. Fungal secondary metabolism: regulation, function and drug discovery. Nature Reviews Microbiology. 2019 Mar;17(3):167-80.
Guerriero G, Berni R, Muñoz-Sanchez JA, Apone F, Abdel-Salam EM, Qahtan AA, et al. Production of plant secondary metabolites: Examples, tips and suggestions for biotechnologists. Genes. 2018 Jun 20;9(6):309.
Atlas RM, Snyder JW. Handbook of media for clinical microbiology. CRC Press; 1995 May 19.
Kundu S, Panda T, Mujiumdar SK, Guha B, Bandyopadhyay KK. Pretreatment of Indian cane molasses for increased production of citric acid. Biotechnol. Bioeng, 1984;26 : 1114-1121.
Chakraborty R, Srinivasan MS, Raghanan SK. Proteases by a new Aspergillus niger during solid substrate fermentation. Journal. Microbiology and Biotechnology.1995;10:17-30.
Rashan IJ, Abed AA, Aziz AA. Further observation on the pharmacological activities of the aqueous extract of Aristolochia bottae stems. Fitoloerapial XIII;1992. 4:350-352.
Hameed A, Hussain SA, Ijaz MU, Ullah S, Muhammad Z, Suleria HA, Song Y. Antioxidant activity of polyphenolic extracts of filamentous fungus Mucor circinelloides (WJ11): Extraction, characterization and storage stability of food emulsions. Food Bioscience. 2020 Apr 1;34:100525.
Perez L, Pauli M, Bazequre P. Antibiotic assay by the agar-well diffusion method. Journal of Actabiology. 1990. 15:113-115 .
Chaturvedi T, Hulkko LS, Fredsgaard M, Thomsen MH. Extraction, isolation, and purification of value-added chemicals from lignocellulosic biomass. Processes. 2022 Sep 2;10(9):1752.
Shafique S, Attia U, Shafique S, Tabassum B, Akhtar N, Naeem A, Abbas Q. Management of mung bean leaf spot disease caused by Phoma herbarum through Penicillium janczewskii metabolites mediated by MAPK signaling cascade. Scientific Reports. 2023 Mar 3;13(1):3606.
Kovanda L, Zhang W, Wei X, Luo J, Wu X, Atwill ER, Vaessen S, Li X, Liu Y. In vitro antimicrobial activities of organic acids and their derivatives on several species of gram-negative and gram-positive bacteria. Molecules. 2019 Oct 19;24(20):3770.
Manikandan A, Anandham R, Madhan S, Raghu R, Krishnamoorthy R, Senthilkumar M. Exploring the Phyllosphere: Microbial Diversity, Interactions, and Ecological Significance in Plant Health. In Plant-Microbe Interaction and Stress Management 2024 Sep 1 (pp. 29-49). Singapore: Springer Nature Singapore.
Ibrahim SS, Ionescu D, Grossart HP. Tapping into fungal potential: Biodegradation of plastic and rubber by potent Fungi. Science of The Total Environment. 2024 Jul 15;934:173188.
Keswani C, Singh HB, Hermosa R, García-Estrada C, Caradus J, He YW, Mezaache-Aichour S, Glare TR, Borriss R, Vinale F, Sansinenea E. Antimicrobial secondary metabolites from agriculturally important fungi as next biocontrol agents. Applied Microbiology and Biotechnology. 2019 Dec;103:9287-303.
Gufe C, Mugabe TN, Makuvara Z, Marumure J, Benard M. In-vitro assessment of the efficacy of herb-herb combinations against multidrug-resistant mastitis-causing bacteria: Staphylococcus aureus and Klebsiella pneumoniae. Cogent Food & Agriculture. 2023 Dec 31;9(1):2187250.
Tang XM, Xie MX, Gou JL, Chen L, Tian JL, Zhang X, Lu YY, Wang HQ. Antibacterial activity of plants in Cirsium: A comprehensive review. Chinese Journal of Integrative Medicine. 2024 Sep;30(9):835-41.
Zhou J, Cai Y, Liu Y, An H, Deng K, Ashraf MA, Zou L, Wang J. Breaking down the cell wall: Still an attractive antibacterial strategy. Frontiers in Microbiology. 2022 Sep 23;13:952633.
Swathi J, Narendra K, Sowjanya KM, Krishna Satya A. Marine fungal metabolites as a rich source of bioactive compounds. African Journal of Biochemistry Research.2013. 7(10) 184-196.
Gül AZ, Selek Ş, Bekiroğlu S, Demirel M, Çakır FB, Uyanık B. Metabolomic profiling in distinct types of leukemia. Turkish J Bioch/Turk Biyokimya Dergisi. 2023 Jan 2;48:238-246.
Ks N, Eswaran J, Nattamai SP B, MA N, Velusamy KM. Organoruthenium (II) complexes featuring pyrazole‐linked Schiff base ligands: Crystal structure, DNA/BSA interactions, cytotoxicity and molecular docking. Applied Organometallic Chemistry. 2019 Mar;33(3):e4751.
HMDB. Sumiki's acid, 2006. http://www.hmdb.ca/metabolites/HMDB02432
Bugni TS, Ireland CM. Marine-derived fungi: a chemically and biologically diverse group of microorganisms. Natural product reports. 2004;21(1):143-63.
Jadulco R, Proksch P, Wray V, Sudarsono, Berg A, Gräfe U. New Macrolides and Furan Carboxylic Acid Derivative from the Sponge-Derived Fungus Cladosporium herbarum. Journal of natural products. 2001 Apr 27;64(4):527-30.
