Biotyping of Acinetobacter baumannii Iraqi Isolates

Abstract

Background: Acinetobacter baumannii is an opportunistic bacterium associated with many nosocomial infections as Acinetobacter calcoaceticus, Acinetobacter nosocomialis, and Acinetobacter pittii. Aim: To determine the Biotyping of Acinebactor baumannii Iraqi Isolates.
Materials and Methods: One hundred Acinetobacter species samples were collected from wound and burns in hospitalized patients in Al-yarmook Teaching Hospital in Baghdad province. Twenty isolates of Acinetobacter baumannii were identified by conventional and molecular methods, the highest rate of the bacterial isolates were 12% from wounds and 8% from burns. All isolates were detected phenotypical, microscopical and biochemical tests and confirmed by ViTEK2 device.
Results: Most of bacterial isolates showed resistant to the antibiotic Cephalexin (CL) (100%), Piperacillin (PRL) (65%), Ceftazidime (CAZ) (60%), Ticarcillin-Clavanic acid (TIM) (75%), Ampicillin-Sulbactum (SAM) (65%), Ceftriaxone (CRO) (60%), Imipenem (IMP) (50%). In contrast, all isolates were found sensitive to Cefotaxime (CTX) (100%). About 90% of Acinetobacter baumannii isolates were positive to biofilm formation, 70% were own efflux Pump, 100% have ability to adhere to the epithelial cells, 60% could extended spectrum β- Lactamase. Molecular subtyping of ITS region or 16S rRNA gene got through RNA 16 amplified the target gene and sequences of PCR products; sequenced data was blasted in NCBI and analysis to construction of rooted phylogenetic tree.
Conclusion: ITS region or 16S rRNA gene was more discriminative than the other gene especially in closely related bacterial isolates, identification and categorization using PCR and sequence technique for 16S rRNA gene is dependable and provides epidemiological importance of Acenetobacter baumannii