INSR and PLIN Polymorphism in Women with Poly Cystic Ovary Syndrome (PCOS) and Its Correlation with Insulin Resistance

Polycystic ovary syndrome

Abstract

Background: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders that affect women of childbearing age. Several genes, in addition to environmental factors, are involved in PCOS events.
Aim: To clarify the association between INSR and PLIN genes and PCOS development.
Materials and methods: SNPs in INSR and PLIN was determined using RFLP- PCR and ARMS.
Results: There was a significant increase in BMI, Triglyceride, VLDL and Sugar (29.22, 154.66, 30.01, 109.83, respectively) in the PCOS
group as compared to the control group (25.19, 121.13, 25.2, 93..4). The frequency of the C allele for INSR in the PCOS group was 0.46,
while it was 0.3 in control group.. TT genotype was associated with increased BMI, Triglyceride, HDL, VLDL and Sugar. The frequency
of GG in the PCOS group was 0.3 compared to 0.17 in the control group. The increase in BMI, Triglyceride, VLDL and Sugar in PCOS
group was associated with the CC genotype of the PLIN gene. However, the menarche age was not significantly different between PCOS and control groups. This study indicated association of SNPs in INSR and PLIN with insulin resistance and lipid metabolism disorders in women with PCOS Conclusion: An association of C/T polymorphism at Exon 17 of INSR with PCOS in women was observed. This indicates that the genotype CT may be a risk factor for developing PCOS. As for the PLIN gene, it was found that the group of healthy women who have the genotype CG (heterozygous asymmetric) is the genotype that is not responsible for the occurrence of the disease when compared with the group of women who have the genotype CC (homozygous symmetry).