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Zainab Baqir Abbas Zainab.22esp15@student.uomosul.edu.iq
Hasan Faisal Hussein Kahya dr.hasankahya@uomosul.edu.iq


Abstract

Abstract


In this study, 15 isolates of Escherichia coli were isolated from patients with urinary tract infections from Al-Khansa'a Teaching Hospital Mosul /Iraq from May 2023 till July 2023. The isolates were studied and diagnosed biochemically. The result of microscopic tests showed isolates that are short Gram-negative bacilli, and the result of biochemical tests was that the isolates were positive for catalase, triple sugar iron, indole, methyl red, coagulation enzyme, fermentation of the following sugars maltose, sucrose, galactose and glucose, while Escherichia coli isolates showed negative results for urease, Voges-Proskauer, and citrate tests. The isolates also showed resistance to all antibiotics used, with high resistance to both ampicillin, erythromycin, and cephalothin, which amounted to 100%. 60% for nalidixic, 53.33% for both azithromycin and levofloxacin and the lowest resistance rate shown by the isolates to gentamicin was 46.7%.

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Abbas, Z. B., & Kahya, H. F. H. (2025). Isolation and Diagnosis of Antibiotic-Resistant Escherichia coli Bacteria from Urinary Tract Infection Patients in Mosul. Al-Kitab Journal for Pure Sciences, 9(01), 172–182. https://doi.org/10.32441/kjps.09.01.p12
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References

References

Walker MM, Roberts JA, Rogers BA, Harris PNA, Sime FB. Current and emerging treatment options for multidrug-resistant Escherichia coli urosepsis: A Review. Antibiotics (Basel). 2022;11(5):456

Zagaglia C, Ammendolia MG, Maurizi L, Nicoletti M, Longhi C. Urinary tract infections caused by uropathogenic Escherichia coli strains—new strategies for an old pathogen. Microorganisms. 2022;10(7),1425.

Lin W-H, Wang M-C, Liu P-Y, Chen P-S, Wen L-L, Teng C-H, et al. Escherichia coli urinary tract infections: host age-related differences in bacterial virulence factors and antimicrobial susceptibility. J Microbiol Immunol Infect. 2022; 55:249–56.

Levinson W. Review of Medical Microbiology and Immunology. 14th ed. New York: McGraw-Hill Education; 2016. p. 821.

Khawcharoenporn T, Vasoo S, Singh K. Urinary Tract Infections due to Multidrug-Resistant Enterobacteriaceae: Prevalence and Risk Factors in a Chicago Emergency department. Emergency medicine international, 2013(1),158517

Poirel L, Madec JY, Lupo A, Schink AK, Kieffer N, Nordmann P, Schwarz S. Antimicrobial Resistance in Escherichia coli. Microbiol Spectr. 2018 Jul;6(4).

Wanger A, Chavez V, Huang R, Wahed A, Dasgupta A, Actor JK. Microbiology and molecular diagnosis in pathology: a comprehensive review for board preparation, certification and clinical practice. 1st edition, 2017.

Procop GW, Church DL, Hall GS, Janda WM. Koneman's color atlas and textbook of diagnostic microbiology. Jones & Bartlett Learning; 2020.

Hemraj V, Diksha S, Avneet G. A review on commonly used biochemical test for bacteria. IJLS. 2013;1(1):1-7.

Brown AE, Smith HR. Benson's Microbiological Applications Laboratory Manual in General Microbiology. 14th ed. New York: McGraw-Hill Higher Education; 2017. 438 p.

Tille PM. Baily and Scott's Diagnostic Microbiology. 14th ed. China: Elsevier Inc.; 2017. 1115 p.

Forbes BA, Saham DF, Weissfeld AS. Baily and Scott’s Diagnostic Microbiology. 12th ed. Mosby Inc., an affiliate of Elsevier Inc.; 2007. 1031 p.

Harley JP, Prescott LM. Laboratory Exercises in Microbiology. 4th ed. McGraw-Hill Education, Inc.; 2002. 466 p.

Mahon CR, Lehman DC, Manuselis G. Textbook of Diagnostic Microbiology. 4th ed. Philadelphia: W.B. Saunders Co.; 2011.

Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing; twenty fifth informational supplement. CLSI Document M100-S31. 2021.

Abdal Jabar RM, Hassoon AH. Study of acrA efflux pump gene in local isolates of Klebsiella pneumoniae exposed to different types of antibiotics. M.Sc. thesis. College Of education for pure sciences/ Ibn Al-Haitham, Department of Biology. Baghdad University, Baghdad, Iraq; 2018.

Al-Saadi ZA. Phenotypic and molecular detection of Escherichia coli efflux pumps from UTI patients. Master Thesis, Education for Pure Sciences, University of Baghdad, Baghdad, Iraq; 2019.

Ait-Mimoune N, Hassaine H, Boulanoir M. Bacteriological profile of urinary tract infections and antibiotic susceptibility of Escherichia coli in Algeria. Iran J Microbiol. 2022;14(2):156-160.

Hashemian H, Saleh ZV, Afzalipoor M, Jafari A. Antibiotic resistance patterns of uropathogenic causes of urinary tract infections in <3-year-old children: a single-center cross-sectional study. Arch Pediatr Infect Dis. 2023;11(3).

Ismael NM, Azzam M, Abdelmoteleb M, El-Shibiny A. Phage vB_Ec_ZCEC14 to treat antibiotic-resistant Escherichia coli isolated from urinary tract infections. Virol J. 2024;21(1):44.

Ramirez-Castillo FY, Moreno-Flores AC, Avelar-González FJ, Márquez-Díaz F, Harel J, Guerrero-Barrera AL. An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: cross-sectional study. Ann Clin Microbiol Antimicrob. 2018;17:1-13. ‏

Ghotaslou R, Baghbani S, Ghotaslou P, Mirmahdavi S, Leylabadlo HE. Molecular epidemiology of antibiotic-resistant Escherichia coli among clinical samples isolated in Azerbaijan, Iran. Iran J Microbiol. 2023;15(3):383.

Zaman SB, Hussain MA, Nye R, Mehta V, Mamun KT, Hossain N. A review on antibiotic resistance: alarm bells are ringing. Cureus. 2017;9(6):1-9.

Salehi A, Davari K, Karimoddini M, Sanani MGP. Evaluating the macrolide resistance of Escherichia coli isolated from urinary infection and determining the phylogeny using the ERIC-PCR method. Eur J Mol Clin Med. 2021;8(1):2113.