Determination of Synergistic Inhibitory Activity of some Nanoparticles and Antibiotic Against Pathogenic Bacteria Caused Acne
Acne and Nanoparticles
Keywords:
Acne, TiO2, Staphylococcus aureus, Citrobacter freundii, Strep. Pyogenes, Ps. aeruginosa, Ps. stutzeri, Enterobacter cloacae, Staph. epidermidis, Staph. saprophyticus, Klebsiella oxytoca, Raoultella ornithinolyticaAbstract
Background: Acne is a common adolescent disease with social impact on affected individual and his family. Many drug formulations available for acne treatment but with varied treatment response and associated with patient none compliance.
Aim: Determine the inhibitory effect of antibiotics and nanoparticles combination against bacteria that cause acne.
Materials and methods: This study was conducted at Laboratories of the Department of Biology/ College of Education for Pure Sciences/ University of Tikrit. 124 samples were collected by swap survey of hospitalized patients with acne in Salahuldean General Hospital during the period from 1/8/2018 to 1/4/2019. After the diagnosis using biochemical tests as well as Vitek2 Compact System, the ability of Gram positive and negative bacterial species that produce virulence factors such as Hemolysin, Urease, Biofilm, Lecithinase, Lipase and β-Lactamase were investigated.
Results: Twelve types of antibiotics were used and the isolates showed different degrees of sensitivity to antibiotics. For the inhibition of titanium dioxide nanoparticles, the results showed that TiO2 nanoparticles had the highest inhibition capacity for Gram-positive bacteria, while the inhibition of Gram-negative species was relatively low. The combination of TiO2 nanoparticles and antibiotic shows inhibitory diameter ranged between 25 to 50 mm against Gran positive bacteria for nano concentration of 50 and 100 mg respectively. While Gram negative bacteria show inhibitory diameter ranged between 20-30 mm for TiO2 nanoparticles concentrations were 50 and 100 mg respectively.
Conclusion: Isolated organisms demonstrated high resistance rate to antibiotics; however, antibiotic combination with TiO2 induced synergistic inhibitory activity against Gram positive and Gram negative bacteria. And the effect was higher in gram positive as compared to gram negative bacteria. This finding suggests new drug formulation for acne treatment.
